![]() ![]() Activation of the human β-glucan receptor dectin-1 correlated with the amount of β-glucan in each fraction. PeWN, containing the 3 -O methylated mannogalactan, was inactive towards TLR2, whereas fraction PeWB, containing more β-glucan, activated the TLR2-TLR6 heterodimer. Affinity for TLR2, TLR2-TLR6 and dectin-1 using HEK-cells expressing the relevant receptor genes was tested. Traces of a (1→3)-α- d-glucan and a (1→2)-α- d-mannan were also observed. In the present study, Pleurotus eryngii polysaccharide fractions containing a 3- O methylated mannogalactan and (1→3)/(1→6)-β- d-glucans were isolated and extensively characterized by 2D NMR and methylation analysis. The mushroom cell wall contains polysaccharides that can activate cells of the innate immune system through receptors such as Toll-like receptors (TLR) and dectin-1.
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